Mbiri ndi Kufunika kwa Kafukufuku
Mu kupanga katemera wa FMD, zinthu zoteteza maselo zimakhala ndi zinyalala zambiri za maselo. Ngati zayikidwa mwachindunji mu kusefa koyeretsera kwa 0.2 μm, ma filter nembanemba amatha kuipitsidwa mwachangu, zomwe zimachepetsa kugwira ntchito bwino komanso zomwe zingakhudze kulowa ndi kutuluka kwa antigen (tinthu ta 146S ta tizilombo toyambitsa matenda).
Phunziroli likuyang'ana mafunso atatu ofunikira:
Kuyerekeza momwe kusefera pamwamba ndi kusefera kwakuya kumagwirira ntchito potengera kuchuluka kwa madzi, kuchepetsa kutayikira kwa madzi, komanso kuteteza zosefera zoyeretsera madzi zomwe zili pansi pa madzi.
Kutsimikizira ubwino wapadera wa zosefera zakuya zomwe zili ndi mphamvu yokwanira pochotsa zinyalala za maselo.
Kukhazikitsa njira zosefera zomwe zingakulitsidwe zomwe zimagwirizana ndi madzi opangidwa ndi matope osiyanasiyana, kuti zikwaniritse zosowa za mafakitale.
Zoyeserera Zachikulu ndi Zotsatira
Kusefera Pamwamba vs. Kusefera Kuzama
Kuyesera:Madzi a katemera wa FMD osagwira ntchito adasefedwa pogwiritsa ntchito nembanemba ya polypropylene ya 0.45 μm ndi mapepala oyezera kuya, kutsatiridwa ndi kusefera koyezera 0.2 μm.
Zomwe zapezeka:
Kutayikira koyambira: 77 NTU.
Kusefa kwa kuya:mphamvu yotulutsa 641 L/m²; kutayikira kwa madzi kwachepetsedwa kufika pa 9.65 NTU (yowala komanso yowala).
Kusefera pamwamba:mphamvu yogwiritsira ntchito mphamvu ya 46 L/m² yokha; kutayirira kunatsala 41 NTU.
Kusefa koyeretsera pansi:Pambuyo posefa mozama, zosefera za 0.2 μm zinali ndi mphamvu yotulutsa ya 655 L/m², poyerekeza ndi 91 L/m² yokha pambuyo posefa pamwamba—kukwera kwa 7.2×.
146S imapanga phindu:Kusefa kwakukulu kwambiri kuposa kusefa pamwamba.
Mapeto:Kuchuluka kwa kusefera kwakuya kunali kokwera ndi 13.9× kuposa kusefera pamwamba, kutayikira kwa filtrate kunali kotsika ndi 76.5%, ndipo nthawi yogwiritsira ntchito fyuluta yoyeretsera inali yowonjezereka kuposa 7×. Kuchokera pamalingaliro onse a mtengo ndi phindu, kusefera kwakuya ndikobwino.
Kusefa kwa Kuzama Kokhala ndi Chaji Yabwino Kwambiri vs. Kusefa kwa Kuzama Kokhala ndi Chaji Yabwino Kwambiri
Kuyesera:Mitundu iwiri ya mapepala oyezera kuya omwe ali ndi kukula kofanana kwa ma pore koma mphamvu yosiyana ya chaji (yabwinobwino poyerekeza ndi yamphamvu) adayesedwa pamadzimadzi a FMD osagwira ntchito.
Zomwe zapezeka:
Ma profiles a throughput ndi pressure anali ofanana.
Kuzama kwabwinobwino kwabwinobwinofyuluta:fyuluta yotayirira 25 NTU.
Kuzama kwamphamvu kwabwinofyuluta:filtrate turbidity 9.65 NTU (38.6% yokha ya yachibadwa).
Mphamvu yolimba ya chaji imachokera ku ma resin ligands apadera omwe amawonjezera kulowetsedwa kwa electrostatic.
Malipoti a mabuku amatsimikizira kuti ma filter amphamvu ozama amathanso kuchotsa mapuloteni a maselo a host (HCP) ndi DNA.
Mapeto:Zosefera zamphamvu zozama bwino zimathandiza kuchepetsa kutayikira kwa madzi (<10 NTU), kuteteza kusefera kwa ultrafiltration/sterilizing pansi pa madzi, ndikuchotsa zinyalala zina zokhudzana ndi ndondomekoyi.
1. Mfundo Zaukadaulo ndi Ubwino wa Kusefa Kozama
Kusefera pamwamba:Amagwira tinthu tating'onoting'ono tomwe tili pamwamba pa nembanemba yokha; makamaka kuphimba pogwiritsa ntchito kukula kwake.
Kusefa kwa kuya:Amachotsa tinthu tating'onoting'ono m'kati mwa matrix pogwiritsa ntchito entrapment ndi electrostatic adsorption.
Ubwino waukulu:
Palibe asbestos (mosiyana ndi zosefera zoyambirira).
Yopangidwa ndi ulusi wa cellulose woyeretsedwa kwambiri, zothandizira zosefera, ndi ma resins a polymeric.
Magulu ogwira ntchito omwe ali ndi mphamvu yabwino amapereka mphamvu yokwanira yopezera tinthu tating'onoting'ono ndi zinthu zosafunika.
Kutsatira kwathunthu zofunikira pa malamulo a biopharmaceutical.
2. Njira Zosefera Zosasinthika pa Magawo Osiyanasiyana a Turbidity
Kutengera maphunziro oyerekeza:
Zochepakutayiriramadzi:kusefa kopepuka kokwanira.
Pakatikatikutayiriramadzi:Kusefa kwa kuya kwa magawo awiri kumalimbikitsidwa.
Pamwambakutayiriramadzi:Kusefa kwamphamvu kwakuya kofunikira kuti muchepetse kutayikira pansi pa NTU 10, kuonetsetsa kuti njira yogwiritsira ntchito bwino pansi pa madzi ndikupereka ma antigen ambiri.
3. Kukhazikitsa Zogulitsa: Zosefera za Great Wall Filtration Depth
Great Wall Filtration imapereka njira zosefera zakuya zomwe zimapangidwira njira zopangira mankhwala.
Zinthu zofunika:
Mapepala oyezera zinthu okhala ndi mphamvu zambiri zochotsera maselo, zinyalala, ndi zinthu zazikulu zolimba.
Imagwiritsidwa ntchito pa maselo a nyama zoyamwitsa, mabakiteriya/ma lysates.
Wokhoza kuchotsa HCP, DNA, mavairasi, endotoxins, ndi mankhwala oletsa thovu.
Kapangidwe kakang'ono, kokhoza kukulitsidwa molunjika, koyenera kugwiritsidwa ntchito pa mzere kapena motsatizana.
Kuthamanga kwa madzi kokhazikika, nthawi yayitali yogwirira ntchito, komanso mtengo wotsika wogwirira ntchito.
Mapeto
Ukadaulo wamphamvu wosefera madzi akuya womwe uli ndi mphamvu zabwino umathandiza kwambiri kumveketsa bwino zinthu komanso kubwezeretsa ma antigen pakupanga katemera wa FMD. Kupatula katemera wa FMD, umapereka njira yodalirika yoyeretsera zinthu zina zamoyo. Pamene kukonza njira za bioprocess kukupitirira, ukadaulo wapamwamba wosefera udzakhala wofunikira kwambiri popanga katemera motetezeka komanso moyenera.
Nkhaniyi yachokera pa deta yeniyeni yoyesera kuti igwiritsidwe ntchito kokha.Kutsimikizirapansi pa mikhalidwe yeniyeni yopangira, akulangizidwa.
